GSM1662335: NHEK-M H3K4me3-2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Epidermis
Cell type
NHEK
Tissue
skin
Lineage
ectoderm
Description
epidermal keratinocytes
Attributes by original data submitter
Sample
source_name
Primary human epidermal keratinocytes (NHEK)
antibody
H3K4me3
antibody vendor/catalog
millipore 07-473
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with the respective histone antibodies. Sequencing libraries were generated for the H3K27ac, H3K4me1, and H3K4me3 samples (samples were divided in half, and two libraries made for each using different multiplexed adaptors) Clustering and 50-cycle single end sequencing were performed on the Illumina Illumina HiSeq 2000 Genome Analyzer. Libraries were sequenced on the Illumina Illumina HiSeq 2000, with 50bp single end sequencing