Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
Yale-m
lab description
Weissman - Yale University
datatype
ChipSeq
datatype description
ChIP-Seq
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-Yale
control description
Input signal from Mouse IgG ChIP-seq prepared at Yale
age
immortalized
age description
Immortal cells
control
IgG-Yale
control description
Input signal from Mouse IgG ChIP-seq prepared at Yale
controlid
CH12/None/Input/IgG-Yale
replicate
1
replicate description
tier 1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM798327: Yale_ChipSeq_CH12_H3K4me3_IgG-Yale
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
53796206
Reads aligned (%)
73.1
Duplicates removed (%)
9.5
Number of peaks
24864 (qval < 1E-05)

mm9

Number of total reads
53796206
Reads aligned (%)
72.9
Duplicates removed (%)
9.5
Number of peaks
24930 (qval < 1E-05)

Base call quality data from DBCLS SRA