Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
ChIP-Seq
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
H3K4me3B
antibody antibodydescription
Rabbit monoclonal antibody
antibody targetdescription
Histone H3 (tri-methyl K4) marks promoters in chromatin regions
antibody vendorname
Cell Signaling
antibody vendorid
9751S
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH000672
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM788087: USC_ChipSeq_K562_H3K4me3B_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
66368466
Reads aligned (%)
76.7
Duplicates removed (%)
33.7
Number of peaks
28693 (qval < 1E-05)

hg19

Number of total reads
66368466
Reads aligned (%)
76.5
Duplicates removed (%)
33.9
Number of peaks
28529 (qval < 1E-05)

Base call quality data from DBCLS SRA