Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
PBMC
Tissue
blood
Lineage
mesoderm
Description
peripheral blood mononuclear cells

Attributes by original data submitter

Sample

source_name
PBMC
biomaterial_provider
PBMC cells provided by E. Bresnick, UW Madison
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
ChIP-Seq
cell
PBMC
cell organism
human
cell description
Peripheral blood mononuclear cells
cell lineage
mesoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
H3K4me3B
antibody antibodydescription
Rabbit monoclonal antibody
antibody targetdescription
Histone H3 (tri-methyl K4) marks promoters in chromatin regions
antibody vendorname
Cell Signaling
antibody vendorid
9751S
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH001883
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM788075: USC_ChipSeq_PBMC_H3K4me3B_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
90199319
Reads aligned (%)
86.8
Duplicates removed (%)
22.9
Number of peaks
22005 (qval < 1E-05)

hg19

Number of total reads
90199319
Reads aligned (%)
86.0
Duplicates removed (%)
24.4
Number of peaks
21947 (qval < 1E-05)

Base call quality data from DBCLS SRA