Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF7L2

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT-116
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
ChIP-Seq
cell
HCT-116
cell organism
human
cell description
colorectal carcinoma (PMID: 7214343)
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
TCF7L2
antibody antibodydescription
Rabbit monoclonal
antibody targetdescription
TCF7L2 (formerly known as TCF4) is a member of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of the following: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4
antibody vendorname
Cell Signaling Technology
antibody vendorid
2569S
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH000627
labversion
Fragmented using Bioruptor, precipitated with protein G magnetic beads,
replicate
1
replicate description
tier 1
softwareversion
PeakSeq1.0 (fdr 0.001)

Sequenced DNA Library

library_name
GSM782123: USC_ChipSeq_HCT-116_TCF4_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
18906254
Reads aligned (%)
89.2
Duplicates removed (%)
6.1
Number of peaks
26911 (qval < 1E-05)

hg38

Number of total reads
18906254
Reads aligned (%)
90.3
Duplicates removed (%)
5.5
Number of peaks
26921 (qval < 1E-05)

Base call quality data from DBCLS SRA