Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Muscle
Cell type
HSMM
Tissue
muscle
Lineage
mesoderm
Description
skeletal muscle myoblasts

Attributes by original data submitter

Sample

source_name
HSMMtube
biomaterial_provider
Lonza
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
skeletal muscle myotubes differentiated from the HSMM cell line
cell lineage
mesoderm
cell sex
U
antibody antibodydescription
Rabbit polyclonal. Antibody Target: CTCF
antibody targetdescription
CTCF zinc finger transcription factor. A sequence specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spreading of chromatin structure in certain instances.
antibody vendorname
Millipore
antibody vendorid
07-729
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001001
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733783: Bernstein_HSMMtube_CTCF
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
46797761
Reads aligned (%)
74.0
Duplicates removed (%)
23.4
Number of peaks
43502 (qval < 1E-05)

hg19

Number of total reads
46797761
Reads aligned (%)
73.5
Duplicates removed (%)
24.5
Number of peaks
43385 (qval < 1E-05)

Base call quality data from DBCLS SRA