Antigen

Antigen Class

Histone

Antigen

H3K9me1

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

biomaterial_provider

ATCC

lab

Broad

lab description

Bernstein - Broad Institute

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell organism

human

cell description

leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC

cell lineage

mesoderm

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K9me1

antibody targetdescription

Histone H3 (mono-methyl K9). Is associated with active and accessible regions. NOTE CONTRAST to H3K9me3 which is associated with repressive heterochromatic state.

antibody vendorname

abcam

antibody vendorid

ab8896

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000052

labversion

Illumina_GA2e and Illumina_GA2x

replicate

1

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM733777: Bernstein_K562_H3K9me1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

48239261

Reads aligned (%)

73.6

Duplicates removed (%)

19.8

Number of peaks

1433 (qval < 1E-05)

hg19

Number of total reads

48239261

Reads aligned (%)

73.1

Duplicates removed (%)

20.7

Number of peaks

1643 (qval < 1E-05)