SRX067489
GSM733738: Bernstein HSMMtube H3K4me3

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Muscle
Cell type
HSMM
Tissue
muscle
Lineage
mesoderm
Description
skeletal muscle myoblasts

Attributes by original data submitter

Sample

source_name
HSMMtube
biomaterial_provider
Lonza
donor_id
ENCDO094AAA
biomaterial_type
in vitro differentiated cells
cell_type
myotube
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
skeletal muscle myotubes differentiated from the HSMM cell line
cell lineage
mesoderm
cell sex
U
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody
H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
1)abcam; 2)Millipore
antibody vendorid
ab8580; 07-473
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001001
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733738: Bernstein_HSMMtube_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
33975115
Reads aligned (%)
80.2
Duplicates removed (%)
13.7
Number of peaks
25075 (qval < 1E-05)

hg19

Number of total reads
33975115
Reads aligned (%)
79.6
Duplicates removed (%)
14.8
Number of peaks
25009 (qval < 1E-05)

Base call quality data from DBCLS SRA