Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K79me2

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
antibody antibodydescription
Rabbit polyclonal antibody raised against a peptide containing K79 di-methylation. Antibody Target: H3K79me2
antibody targetdescription
H3K79me2 is a mark of the transcriptional transition region - the region between the initiation marks (K4me3, etc) and the elongation marks (K36me3).
antibody vendorname
Active Motif
antibody vendorid
39143
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000037
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733736: Bernstein_GM12878_H3K79me2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
38796366
Reads aligned (%)
81.7
Duplicates removed (%)
6.9
Number of peaks
10421 (qval < 1E-05)

hg19

Number of total reads
38796366
Reads aligned (%)
81.5
Duplicates removed (%)
7.0
Number of peaks
10714 (qval < 1E-05)

Base call quality data from DBCLS SRA