Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HUVEC
biomaterial_provider
Lonza
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
umbilical vein endothelial cells
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me2
antibody targetdescription
Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters.
antibody vendorname
abcam
antibody vendorid
ab7766
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000060
labversion
Illumina_GA2e and Illumina_GA2x
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733683: Bernstein_HUVEC_H3K4me2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
45408331
Reads aligned (%)
55.0
Duplicates removed (%)
6.5
Number of peaks
54492 (qval < 1E-05)

hg19

Number of total reads
45408331
Reads aligned (%)
54.7
Duplicates removed (%)
7.0
Number of peaks
54374 (qval < 1E-05)

Base call quality data from DBCLS SRA