Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
cell karyotype
normal
cell lineage
mesoderm
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Specific for histone H3 tri methylated at lysine 36, weakly reacts with H3K36me2. Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
abcam
antibody vendorid
ab9050
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000037
labversion
Illumina_GA2e and Illumina_GA2x
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733679: Bernstein_GM12878_H3K36me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
36623663
Reads aligned (%)
71.2
Duplicates removed (%)
5.2
Number of peaks
274 (qval < 1E-05)

hg19

Number of total reads
36623663
Reads aligned (%)
70.7
Duplicates removed (%)
5.9
Number of peaks
307 (qval < 1E-05)

Base call quality data from DBCLS SRA