Antigen

Antigen Class

Histone

Antigen

H3K9ac

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Broad

lab description

Bernstein - Broad Institute

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K9ac

antibody targetdescription

Histone H3 (acetyl K9). As with H3K27ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.

antibody vendorname

abcam

antibody vendorid

ab4441

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000037

labversion

Illumina_GA2e and Illumina_GA2x

replicate

1,2

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM733677: Bernstein_GM12878_H3K9ac

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

27534541

Reads aligned (%)

73.1

Duplicates removed (%)

7.4

Number of peaks

21149 (qval < 1E-05)

hg19

Number of total reads

27534541

Reads aligned (%)

72.6

Duplicates removed (%)

7.8

Number of peaks

21880 (qval < 1E-05)