Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K79me2

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell lineage
mesoderm
antibody antibodydescription
Rabbit polyclonal antibody raised against a peptide containing K79 di-methylation. Antibody Target: H3K79me2
antibody targetdescription
H3K79me2 is a mark of the transcriptional transition region - the region between the initiation marks (K4me3, etc) and the elongation marks (K36me3).
antibody vendorname
Active Motif
antibody vendorid
39143
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000052
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733653: Bernstein_K562_H3K79me2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
52935859
Reads aligned (%)
79.5
Duplicates removed (%)
9.0
Number of peaks
14268 (qval < 1E-05)

hg19

Number of total reads
52935859
Reads aligned (%)
79.4
Duplicates removed (%)
9.2
Number of peaks
14305 (qval < 1E-05)

Base call quality data from DBCLS SRA