Antigen

Antigen Class

Histone

Antigen

H4K20me1

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

lab

Broad

lab description

Bernstein - Broad Institute

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell organism

human

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

cell karyotype

normal

cell lineage

mesoderm

antibody antibodydescription

rabbit polyclonal. Antibody Target: H4K20me1

antibody targetdescription

Histone H4 (mono-methyl K20). Is associated with active and accessible regions. In mammals, PR-Set7 specifically catalyzes H4K20 monomethylation. NOTE CONTRAST to H3K20me3 which is associated with heterochromatin and DNA repair.

antibody vendorname

abcam

antibody vendorid

ab9051

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000037

labversion

Illumina_GA2e and Illumina_GA2x

replicate

1,2

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM733642: Bernstein_GM12878_H4K20me1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

32365639

Reads aligned (%)

68.3

Duplicates removed (%)

3.9

Number of peaks

925 (qval < 1E-05)

hg19

Number of total reads

32365639

Reads aligned (%)

67.8

Duplicates removed (%)

4.5

Number of peaks

1053 (qval < 1E-05)