Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
nhr-28

Cell type

Cell type Class
Larvae
Cell type
L3
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_NHR-28_GFP_L3_rep1 extraction3_seq3 channel_1
strain
OP317(official name : OP317 genotype : unc119(ed3); wgIs317(nhr-28::TY1 EGFP FLAG; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-28::EGFP fusion protein is expressed in the correct nhr-28 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-28 transcription factor. made_by : R Waterston )
developmental stage
L3
genotype
unc119(ed3);wgIs317(nhr-28::TY1 EGFP FLAG;unc119)
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM729390: Snyder_NHR-28_GFP_L3_rep1 extraction3_seq3 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
8960112
Reads aligned (%)
25.6
Duplicates removed (%)
7.9
Number of peaks
322 (qval < 1E-05)

ce10

Number of total reads
8960112
Reads aligned (%)
25.6
Duplicates removed (%)
7.9
Number of peaks
323 (qval < 1E-05)

Base call quality data from DBCLS SRA