Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
daf-12

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_DAF-12_GFP_L4_rep1 extraction3_seq3 channel_1
strain
OP222(official name : OP222 genotype : unc119(ed3); wgIs222(daf-12:TY1 EGFP FLAG; unc119) outcross : 3 transgene : daf-12 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The DAF-12::EGFP fusion protein is expressed in the correct daf-12 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DAF-12 transcription factor. made_by : Mihail Sarov )
developmental stage
L4
genotype
unc119(ed3);wgIs222(daf-12:TY1 EGFP FLAG;unc119)
Sex
hermaphrodite
transgene
daf-12

Sequenced DNA Library

library_name
GSM729370: Snyder_DAF-12_GFP_L4_rep1 extraction3_seq3 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
5788678
Reads aligned (%)
29.4
Duplicates removed (%)
9.3
Number of peaks
232 (qval < 1E-05)

ce10

Number of total reads
5788678
Reads aligned (%)
29.4
Duplicates removed (%)
9.3
Number of peaks
230 (qval < 1E-05)

Base call quality data from DBCLS SRA