Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Larvae
Cell type
L1
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_ALY-2_Input_L1 extraction1_seq1 channel_1
strain
OP217(official name : OP217 genotype : unc119(ed3); wgIs217(aly-2:TY1 EGFP FLAG; unc119) outcross : 3 transgene : aly-2 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ALY-2::EGFP fusion protein is expressed in the correct aly-2 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ALY-2 transcription factor. made_by : Mihail Sarov )
developmental stage
fed L1
genotype
unc119(ed3);wgIs217(aly-2:TY1 EGFP FLAG;unc119)
Sex
hermaphrodite
transgene
aly-2

Sequenced DNA Library

library_name
GSM729380: Snyder_ALY-2_Input_L1 extraction1_seq1 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
4678120
Reads aligned (%)
30.9
Duplicates removed (%)
4.8
Number of peaks
324 (qval < 1E-05)

ce10

Number of total reads
4678120
Reads aligned (%)
30.9
Duplicates removed (%)
4.8
Number of peaks
324 (qval < 1E-05)

Base call quality data from DBCLS SRA