Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
zag-1

Cell type

Cell type Class
Larvae
Cell type
L2
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_ZAG-1_GFP_L2_rep2 extraction4_seq4 channel_1
strain
OP83(official name : OP83 genotype : unc119(ed3); wgIs83(zag-1::TY1 EGFP FLAG; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ZAG-1::EGFP fusion protein is expressed in the correct zag-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ZAG-1 transcription factor. made_by : R Waterston )
developmental stage
L2
genotype
unc119(ed3);wgIs83(zag-1::TY1 EGFP FLAG;unc119)
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM729319: Snyder_ZAG-1_GFP_L2_rep2 extraction4_seq4 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
4851771
Reads aligned (%)
34.4
Duplicates removed (%)
4.7
Number of peaks
939 (qval < 1E-05)

ce10

Number of total reads
4851771
Reads aligned (%)
34.4
Duplicates removed (%)
4.7
Number of peaks
937 (qval < 1E-05)

Base call quality data from DBCLS SRA