Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

Osteosarcome cell line

cell line

U2OS

genotype/variation

stably expressing shKAP1 and FLAG-KAP1 wild type

harvest date

2008-12-10

chip antibody

mouse anti-FLAG (Sigma, catalog # F1804)

Sequenced DNA Library

library_name

GSM700368: FLAG-KAP1 wild type_U2OS stables_ChIP-seq_rep2A_lane5

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

41424702

Reads aligned (%)

82.7

Duplicates removed (%)

4.6

Number of peaks

2561 (qval < 1E-05)

hg19

Number of total reads

41424702

Reads aligned (%)

82.1

Duplicates removed (%)

5.6

Number of peaks

932 (qval < 1E-05)