Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TRIM28

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
Osteosarcoma cell line
cell line
U2OS
genotype/variation
stably expressing shKAP1 and FLAG-KAP1 wild type
harvest date
2008-12-10
chip antibody
rabbit anti-KAP1 (Abcam, catalog # ab10483)

Sequenced DNA Library

library_name
GSM700366: FLAG-KAP1 wild type_U2OS stables_ChIP-seq_rep1_lane8
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
38773701
Reads aligned (%)
69.9
Duplicates removed (%)
12.6
Number of peaks
12089 (qval < 1E-05)

hg38

Number of total reads
38773701
Reads aligned (%)
71.0
Duplicates removed (%)
11.8
Number of peaks
12267 (qval < 1E-05)

Base call quality data from DBCLS SRA