Antigen

Antigen Class

TFs and others

Antigen

TRIM28

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

Osteosarcoma cell line

cell line

U2OS

genotype/variation

stably expressing shKAP1 and FLAG-KAP1 wild type

harvest date

2008-12-10

chip antibody

rabbit anti-KAP1 (Abcam, catalog # ab10483)

Sequenced DNA Library

library_name

GSM700366: FLAG-KAP1 wild type_U2OS stables_ChIP-seq_rep1_lane8

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

38773701

Reads aligned (%)

79.9

Duplicates removed (%)

12.6

Number of peaks

12089 (qval < 1E-05)

hg38

Number of total reads

38773701

Reads aligned (%)

80.4

Duplicates removed (%)

11.8

Number of peaks

12267 (qval < 1E-05)