Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pes-1

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_PES-1_GFP_L4_rep2 extraction7_seq7 channel_1
strain
OP87(official name : OP87 genotype : unc-119(ed3) III; wgIs87 [unc-119(+) pes-1::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PES-1::EGFP fusion protein is expressed in the correct pes-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PES-1 transcription factor. made_by : R. Waterston )
developmental stage
L4
genotype
unc-119(ed3) III; wgIs87 [unc-119(+) pes-1::TY1::EGFP::3xFLAG]
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM700174: Snyder_PES-1_GFP_L4_rep2 extraction7_seq7 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
1060761
Reads aligned (%)
29.7
Duplicates removed (%)
5.0
Number of peaks
105 (qval < 1E-05)

ce10

Number of total reads
1060761
Reads aligned (%)
29.7
Duplicates removed (%)
5.0
Number of peaks
105 (qval < 1E-05)

Base call quality data from DBCLS SRA