Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pha-4

Cell type

Cell type Class
Larvae
Cell type
L2
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_PHA-4_GFP_L2_rep1 extraction5_seq5 channel_1
strain
OP37(official name : OP37 genotype : unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PHA-4::EGFP fusion protein is expressed in the correct pha-4 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PHA-4 transcription factor. made_by : R. Waterston and S. Kim )
developmental stage
L2
genotype
unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG]
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM700181: Snyder_PHA-4_GFP_L2_rep1 extraction5_seq5 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
3573958
Reads aligned (%)
5.9
Duplicates removed (%)
2.5
Number of peaks
11 (qval < 1E-05)

ce10

Number of total reads
3573958
Reads aligned (%)
5.9
Duplicates removed (%)
2.5
Number of peaks
11 (qval < 1E-05)

Base call quality data from DBCLS SRA