Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pha-4

Cell type

Cell type Class
Adult
Cell type
Young adult
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_PHA-4_GFP_YA_rep2 extraction4_seq4 channel_1
strain
OP37(official name : OP37 genotype : unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PHA-4::EGFP fusion protein is expressed in the correct pha-4 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PHA-4 transcription factor. made_by : R. Waterston and S. Kim )
developmental stage
Young Adult
genotype
unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG]
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM700194: Snyder_PHA-4_GFP_YA_rep2 extraction4_seq4 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
857311
Reads aligned (%)
59.5
Duplicates removed (%)
13.5
Number of peaks
151 (qval < 1E-05)

ce10

Number of total reads
857311
Reads aligned (%)
59.5
Duplicates removed (%)
13.5
Number of peaks
149 (qval < 1E-05)

Base call quality data from DBCLS SRA