OP201(made_by : S Kim description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PQM-1::EGFP fusion protein is expressed in the correct pqm-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PQM-1 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc119(ed3); wgIs201(pqm-1::TY1 EGFP FLAG C; unc119) official name : OP201 )
developmental stage
L3
genotype
unc119(ed3);wgIs201(pqm-1::TY1 EGFP FLAG C;unc119)