Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
egl-27

Cell type

Cell type Class
Larvae
Cell type
L1
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_EGL-27_GFP_L1_rep2 extraction2_seq1 channel_1
strain
OP177(made_by : S Kim description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The EGL-27::EGFP fusion protein is expressed in the correct egl-27 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the EGL-27 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc119(ed3); wgIs177(egl-27::TY1 EGFP FLAG; unc119) official name : OP177 )
developmental stage
fed L1
genotype
unc119(ed3);wgIs177(egl-27::TY1 EGFP FLAG;unc119)
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM677689: Snyder_EGL-27_GFP_L1_rep2 extraction2_seq1 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
5195866
Reads aligned (%)
44.4
Duplicates removed (%)
6.8
Number of peaks
185 (qval < 1E-05)

ce10

Number of total reads
5195866
Reads aligned (%)
44.4
Duplicates removed (%)
6.8
Number of peaks
185 (qval < 1E-05)

Base call quality data from DBCLS SRA