Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ceh-30

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_CEH-30_GFP_lemb_rep2 extraction2_seq1 channel_2
strain
OP120(made_by : R Waterston description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The CEH-30::EGFP fusion protein is expressed in the correct ceh-30 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the CEH-30 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc119(ed3); wgIs120(ceh-30::TY1 EGFP FLAG; unc119) official name : OP120 )
developmental stage
late embryo
genotype
unc119(ed3);wgIs120(ceh-30::TY1 EGFP FLAG;unc119)
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM677686: Snyder_CEH-30_GFP_lemb_rep2 extraction2_seq1 aliquote 2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
4786851
Reads aligned (%)
90.8
Duplicates removed (%)
6.5
Number of peaks
591 (qval < 1E-05)

ce10

Number of total reads
4786851
Reads aligned (%)
90.8
Duplicates removed (%)
6.5
Number of peaks
592 (qval < 1E-05)

Base call quality data from DBCLS SRA