Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pha-4

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_PHA4_GFP_lemb_rep2 extraction2_seq1 channel_2
strain
OP37(made_by : R. Waterston and S. Kim description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The PHA-4::EGFP fusion protein is expressed in the correct pha-4 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the PHA-4 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG] official name : OP37 )
developmental stage
late embryo
genotype
unc-119(ed3) III; wgIs37 [unc-119(+) pha-4::TY1::EGFP::3xFLAG]
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM677658: Snyder_PHA4_GFP_lemb_rep2 extraction2_seq1 aliquote 2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce10

Number of total reads
4078616
Reads aligned (%)
70.6
Duplicates removed (%)
4.7
Number of peaks
210 (qval < 1E-05)

ce11

Number of total reads
4078616
Reads aligned (%)
70.7
Duplicates removed (%)
4.7
Number of peaks
210 (qval < 1E-05)

Base call quality data from DBCLS SRA