Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
hlh-1

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_HLH-1_GFP_emb_rep1 extraction1_seq1 channel_2
strain
OP64(made_by : R. Waterston description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The HLH-1::EGFP fusion protein is expressed in the correct hlh-1 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the HLH-1 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc-119(ed3) III; wgIs64 [unc-119(+) hlh-1::TY1::EGFP::3xFLAG] official name : OP64 )
developmental stage
embryo
genotype
unc-119(ed3) III; wgIs64 [unc-119(+) hlh-1::TY1::EGFP::3xFLAG]
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM677612: Snyder_HLH-1_GFP_emb_rep1 extraction1_seq1 aliquote 2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce10

Number of total reads
1320973
Reads aligned (%)
75.7
Duplicates removed (%)
3.7
Number of peaks
278 (qval < 1E-05)

ce11

Number of total reads
1320973
Reads aligned (%)
75.8
Duplicates removed (%)
3.7
Number of peaks
277 (qval < 1E-05)

Base call quality data from DBCLS SRA