Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Larvae
Cell type
L3
NA
NA

Attributes by original data submitter

Sample

source_name
Snyder_MAB5_POLII_L3_rep2 extraction2_seq1 channel_1
strain
OP26(made_by : R. Waterston and S. Kim description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The MAB-5::EGFP fusion protein is expressed in the correct mab-5 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the MAB-5 transcription factor. tags : GFP::3xFlag mutagen : Bombard outcross : 3 genotype : unc-119(ed3) III; wgIs26 [unc-119(+) mab-5::TY1::EGFP::3xFLAG] official name : OP26 )
developmental stage
L3
genotype
unc-119(ed3) III; wgIs26 [unc-119(+) mab-5::TY1::EGFP::3xFLAG]
Sex
hermaphrodite

Sequenced DNA Library

library_name
GSM677601: Snyder_MAB5_POLII_L3_rep2 extraction2_seq1 aliquote 1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

ce11

Number of total reads
4001586
Reads aligned (%)
54.7
Duplicates removed (%)
3.9
Number of peaks
164 (qval < 1E-05)

ce10

Number of total reads
4001586
Reads aligned (%)
54.7
Duplicates removed (%)
3.9
Number of peaks
164 (qval < 1E-05)

Base call quality data from DBCLS SRA