Antigen

Antigen Class

TFs and others

Antigen

Stat5a

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

primary CD4+ T cells from spleen and lymph nodes

strain

C57BL/6J

genotype/variation

wild type

cell type

in vitro polarized T helper17 cells

passages

FACS sorted naive CD4+T cells cultured in vitro for 3 days under Th17 condition and restimulated with IL-2 for 30 minutes

chip antibody

mixture of STAT5a (PA-ST5A: R&D) and STAT5b (PA-ST5B: R&D) antibodies, 2 ul each per IP

Sequenced DNA Library

library_name

GSM652878: WTTh17STAT5 (ChIP-Seq)

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

37908454

Reads aligned (%)

93.3

Duplicates removed (%)

39.4

Number of peaks

63651 (qval < 1E-05)

mm9

Number of total reads

37908454

Reads aligned (%)

93.2

Duplicates removed (%)

39.4

Number of peaks

65990 (qval < 1E-05)