Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Stat3

Cell type

Cell type Class
Blood
Cell type
Th1 Cells
MeSH Description
A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Attributes by original data submitter

Sample

source_name
primary CD4+ T cells from spleen and lymph nodes
strain
C57BL/6J
genotype/variation
wild type
cell type
in vitro polarized T helper17 cells
passages
FACS sorted naive CD4+T cells cultured in vitro for 3 days under Th17 condition and restimulated with IL-6 for 30 minutes
chip antibody
5 ug of STAT3 (14-6727: eBioScience) per IP

Sequenced DNA Library

library_name
GSM652877: WTTh17STAT3 (ChIP-Seq)
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
36766113
Reads aligned (%)
93.0
Duplicates removed (%)
25.8
Number of peaks
54722 (qval < 1E-05)

mm9

Number of total reads
36766113
Reads aligned (%)
92.9
Duplicates removed (%)
25.9
Number of peaks
54775 (qval < 1E-05)

Base call quality data from DBCLS SRA