Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

Cell line

strain

U2OS-ERalpha (stable)

antibody

normal rabbit IgG

Sequenced DNA Library

library_name

GSM641430: ChIP_IgG

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

12825873

Reads aligned (%)

76.9

Duplicates removed (%)

12.0

Number of peaks

525 (qval < 1E-05)

hg19

Number of total reads

12825873

Reads aligned (%)

76.2

Duplicates removed (%)

13.7

Number of peaks

774 (qval < 1E-05)