Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
Chromatin IP against p53-pS15
cell line
U2OS
cell type
osteosarcoma
antibody
α-p53-pS15 (Cell Signaling)
compound
Actinomycin D
dose
5 nM

Sequenced DNA Library

library_name
GSM545809: p53-pS15_ActD_ChIPSeq
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
11993970
Reads aligned (%)
70.5
Duplicates removed (%)
4.1
Number of peaks
2052 (qval < 1E-05)

hg38

Number of total reads
11993970
Reads aligned (%)
71.2
Duplicates removed (%)
2.8
Number of peaks
1574 (qval < 1E-05)

Base call quality data from DBCLS SRA