Sample information curated by ChIP-Atlas

Antigen

Antigen Class
No description
Antigen
NA

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

ENA first public
2014-09-19
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2337952
INSDC center alias
HEIDELBERG UNIVERSITY
INSDC center name
HEIDELBERG UNIVERSITY
INSDC first public
2014-09-19T17:02:30Z
INSDC last update
2018-03-08T17:08:39Z
INSDC status
public
Submitter Id
E-MTAB-2228:Olig2
broker name
ArrayExpress
cell line
NS5
cell type
neural stem cells
common name
house mouse
genotype
wild type
sample name
E-MTAB-2228:Olig2

Sequenced DNA Library

library_name
Olig2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Neural stem cells cultured in proliferation-promoting medium Chromatin Immunoprecipitation with antibodies to Olig2 (Millipore, AB9610), Ascl1 (BD Pharmingen, 556604), Tcf3 (Santa Cruz, sc-349), Max (Santa Cruz, sc-197), NFI (Santa Cruz, sc-30918), Sox2 (Santa Cruz sc-17320), Sox9 (Millipore, AB5535) and Sox21 (R&D systems, AF3538) transcription factors. Libraries were prepared from 10ng of ChIP-enriched DNA using Illumina's ChIP-seq DNA sample preparation kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
43997962
Reads aligned (%)
67.5
Duplicates removed (%)
41.3
Number of peaks
51083 (qval < 1E-05)

mm9

Number of total reads
43997962
Reads aligned (%)
67.4
Duplicates removed (%)
41.4
Number of peaks
51204 (qval < 1E-05)

Base call quality data from DBCLS SRA