Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

ENA first public

2019-12-08

ENA last update

2019-12-05

ENA-CHECKLIST

ERC000011

External Id

SAMEA6367954

INSDC center alias

EPFL

INSDC center name

EPFL

INSDC first public

2019-12-08T17:04:28Z

INSDC last update

2019-12-05T12:00:59Z

INSDC status

public

Submitter Id

E-MTAB-8553:FloChIP CEBPB rep2

broker name

ArrayExpress

cell line

GM12878

common name

human

disease

normal

organism part

blood

sample name

E-MTAB-8553:FloChIP CEBPB rep2

Sequenced DNA Library

library_name

FloChIP CEBPB rep2_p

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell line used is GM12878 while the antibodies used are anti-RUNX3 sc-101553, anti-PU.1 sc-390405, anti-C/EBPβ sc-7962 from Santa Cruz. Cell line harvesting (detailes in the MS) Not applicable Not applicable Not applicable Not applicable Qiagen kit (detailes in the MS) Nextera library prep (detailes in the MS)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

41793298

Reads aligned (%)

93.9

Duplicates removed (%)

49.8

Number of peaks

970 (qval < 1E-05)

hg19

Number of total reads

41793298

Reads aligned (%)

77.9

Duplicates removed (%)

48.5

Number of peaks

975 (qval < 1E-05)