The Nuffield Department of Medicine, University of Oxford, Oxford, UK
INSDC center name
The Nuffield Department of Medicine, University of Oxford, Oxford, UK
INSDC first public
2014-02-10T17:00:48Z
INSDC last update
2018-03-08T16:52:56Z
INSDC status
public
Submitter Id
E-MTAB-1995:cell_culture_Hypoxia_RNA-Pol2
broker name
ArrayExpress
cell line
MCF-7
common name
human
sample name
E-MTAB-1995:cell_culture_Hypoxia_RNA-Pol2
Sequenced DNA Library
library_name
cell_culture_Hypoxia_RNA-Pol2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MCF-7 cells were cultured in Dulbeccos modified Eagle's medium 'DMEM' (Invitrogen). Media were supplemented with and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich). Cells were incubated for 24 hours in an In vivo2 Hypoxia Work Station (Ruskin Technologies) in an atmosphere of either normoxia (21% oxygen) or hypoxia (0.5% oxygen). Chromatin immunoprecipitation was performed as described in the paper. Libraries were prepared using the Illumina ChIP-Seq kit.