Sample information curated by ChIP-Atlas

Antigen

Antigen Class
DNase-seq
Antigen
DNase-Seq

Cell type

Cell type Class
Blood
Cell type
DLBCL
NA
NA

Attributes by original data submitter

Sample

DISEASE_ONTOLOGY_URI
C1333295
ENA first public
2014-08-12
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2237204
GENETIC_CHARACTERISTICS
t(14;18) negative
INSDC center alias
NCMLS
INSDC center name
Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:05Z
INSDC last update
2018-03-08T16:50:46Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://www.ebi.ac.uk/efo/EFO_0002356
Submitter Id
SU-DHL-5_d01
biomaterial provider
DSMZ
biomaterial type
Cell Line
common name
human
differentiation method
Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011
differentiation stage
B cell
disease
Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma
line
SU-DHL-5
medium
RPMI 1640 + 10%FBS + 1% Glutamine
molecule
total RNA
sample name
SU-DHL-5_d01
sex
female

Sequenced DNA Library

library_name
BlueprintWP4_SuDHL5_DNaseI_100U_18M_starting cells
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
SuDHL5 nuclei were isolated and briefly treated with different concentrations of DNaseI enzyme. Based on quality check 100U DNase treated sample was further processed with sucrose fractionation and purified sample was passed to the illumina library prep

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
25757472
Reads aligned (%)
89.5
Duplicates removed (%)
23.8
Number of peaks
22680 (qval < 1E-05)

hg19

Number of total reads
25757472
Reads aligned (%)
88.7
Duplicates removed (%)
25.3
Number of peaks
22723 (qval < 1E-05)

Base call quality data from DBCLS SRA