Illumina HiSeq 2000 sequencing; DNase-Hypersensitivity Diffuse large B cell lymphoma GCB
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Blood
Cell type
DLBCL
NA
NA
Attributes by original data submitter
Sample
DISEASE_ONTOLOGY_URI
C1333295
ENA first public
2014-08-12
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2237206
GENETIC_CHARACTERISTICS
t(14;18)-positive
INSDC center alias
NCMLS
INSDC center name
Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:05Z
INSDC last update
2018-03-08T16:50:46Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://purl.obolibrary.org/obo/CLO_0007066
Submitter Id
KARPAS-422_d01
biomaterial provider
DSMZ
biomaterial type
Cell Line
common name
human
differentiation method
Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011
differentiation stage
B cell
disease
Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma
line
KARPAS-422
medium
RPMI 1640 + 20%FBS + 1% Glutamine
molecule
genomic DNA
sample name
KARPAS-422_d01
sex
female
Sequenced DNA Library
library_name
BlueprintWP4_Karpas_DNaseI_80U_18M_starting cells
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
Karpas nuclei were isolated and briefly treated with different concentrations of DNaseI enzyme. Based on quality check 80U DNase treated sample was further processed with sucrose fractionation and purified sample was passed to the illumina library prep