Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:05Z
INSDC last update
2018-03-08T16:51:00Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://purl.obolibrary.org/obo/CLO_0007059
Submitter Id
K-562-d01
biomaterial provider
ATCC
biomaterial type
Cell Line
common name
human
differentiation method
erythroleukemia type, CML patient, positive for the bcr:abl fusion gene
disease
Chronic Myelogenous Leukemia
line
K-562
lineage
mesoderm
medium
RPMI1640 + glutamax 10% FCS and 1% pen/strep
molecule
genomic DNA
passage
+/- 11 after obtaining cells from ATCC
sample name
K-562-d01
sex
female
Sequenced DNA Library
library_name
K562_DNaseI_80U_Molbio_5M_startingcells
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
K562 cell line nuclei were isolated and briefly treated with different concentrations of DNaseI enzyme. Based on quality check 80U DNase treated sample was further processed with sucrose fractionation and purified sample was passed to the illumina library