NextSeq 500 paired end sequencing; ChIP-seq against BZLF1 in Raji cells (EBV, Human Herpesvirus 4, GenBank accession KF717093).
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Blood
Cell type
RAJI
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type
Attributes by original data submitter
Sample
ENA first public
2019-04-01
ENA last update
2019-03-18
ENA-CHECKLIST
ERC000011
External Id
SAMEA5425656
INSDC center alias
Research Unit Gene Vectors Helmholtz Zentrum Munchen German Research Center for Environmental Health and German Center for Infection Research (DZIF), Partner site Munich, Germany
INSDC center name
Research Unit Gene Vectors Helmholtz Zentrum Munchen German Research Center for Environmental Health and German Center for Infection Research (DZIF), Partner site Munich, Germany
INSDC first public
2019-04-01T17:02:00Z
INSDC last update
2019-03-18T13:32:44Z
INSDC status
public
Submitter Id
E-MTAB-7788:Replicate 2 Raji iBZLF1 full-length input high
broker name
ArrayExpress
cell line
Raji
cell type
B-lymphoblast
common name
human
replicate
biological replicate
sample name
E-MTAB-7788:Replicate 2 Raji iBZLF1 full-length input high
stimulus
doxycycline (100 ng/ml)
Sequenced DNA Library
library_name
Replicate 2 Raji iBZLF1 full-length input high_p
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1x10^8 Raji iBZLF1 cells were spun down (400 g, 7 min, RT) and washed with PBS. 1 x 10^8 cells were adjusted to a concentration of 5 x 10^5 cells/ ml before chromatin preparation. Detailed protocols can be found in the most recent version of the publicly available manuscript (https://www.biorxiv.org/content/10.1101/317354v1). The library construction was performed by the company Vertis Biotechnology AG (Freising, Germany) using TrueSeq sequencing adaptors and DNA fragments of 350 to 600 nt in length.