Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PRDM1

Cell type

Cell type Class
Blood
Cell type
NCI-H929
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

Alias
E-MTAB-7739:NCI-H929 BLIMP1 ChIP-2
Broker name
ArrayExpress
Description
Protocols: Chromatin immunoprecipitation was performed on formaldehyde-crosslinked cells. ChIP DNA was extracted with phenol-chloroform. Sequencing libraries were prepared from ChIP DNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (#E7370S, New England Biolabs) with the Multiplex Oligos for Illumina (#E7335S and #E7500S, New England Biolabs).
ENA checklist
ERC000011
INSDC center alias
Department of Anatomy, University of Iceland
INSDC center name
Department of Anatomy, University of Iceland
INSDC first public
2019-05-08T04:04:30Z
INSDC last update
2019-03-05T15:38:45Z
INSDC status
public
SRA accession
ERS3202509
Sample Name
ERS3202509
Title
NCI-H929 BLIMP1 ChIP-2
cell_line
NCI-H929
cell_type
plasma cell
developmental stage
adult
disease
multiple myeloma
genotype
wild type genotype
organism
Homo sapiens
organism part
bone marrow

Sequenced DNA Library

library_name
NCI-H929 BLIMP1 ChIP-2_p
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed on formaldehyde-crosslinked cells. ChIP DNA was extracted with phenol-chloroform. Sequencing libraries were prepared from ChIP DNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (#E7370S, New England Biolabs) with the Multiplex Oligos for Illumina (#E7335S and #E7500S, New England Biolabs).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
35877321
Reads aligned (%)
182.0
Duplicates removed (%)
2.3
Number of peaks
801 (qval < 1E-05)

hg38

Number of total reads
35877321
Reads aligned (%)
184.6
Duplicates removed (%)
1.9
Number of peaks
1388 (qval < 1E-05)

Base call quality data from DBCLS SRA