Illumina HiSeq 2500 paired end sequencing; RNAseq of human cell line RPCI-WM1 comparing artificial PRDM1-targeting miRNA to non-targeting control, and comparing Tazemetostat treatment to DMSO. ChIPseq for the factors BLIMP1 and H3K27me3 in the RPCI-WM1, OPM-2 and NCI-H929 cell lines, along with ChIPseq for EZH2 in the NCI-H929 cell line.
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Blood
Cell type
RPCI-WM1
NA
NA
Attributes by original data submitter
Sample
ENA first public
2019-05-08
ENA last update
2019-03-05
ENA-CHECKLIST
ERC000011
External Id
SAMEA5396139
INSDC center alias
Department of Anatomy, University of Iceland
INSDC center name
Department of Anatomy, University of Iceland
INSDC first public
2019-05-08T04:04:30Z
INSDC last update
2019-03-05T15:38:44Z
INSDC status
public
Submitter Id
E-MTAB-7739:RPCI-WM1 H3-2
broker name
ArrayExpress
cell line
RPCI-WM1
cell type
lymphocyte
common name
human
developmental stage
adult
disease
Waldenstrom macroglobulinemia
genotype
wild type genotype
organism part
lymph node
sample name
E-MTAB-7739:RPCI-WM1 H3-2
Sequenced DNA Library
library_name
RPCI-WM1 H3-2_p
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed on formaldehyde-crosslinked cells. ChIP DNA was extracted with phenol-chloroform. Sequencing libraries were prepared from ChIP DNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (#E7370S, New England Biolabs) with the Multiplex Oligos for Illumina (#E7335S and #E7500S, New England Biolabs).