Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Blood

Cell type

Multiple Myeloma

MeSH Description

A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Sample

DISEASE_ONTOLOGY_URI

C0026764

ENA first public

2014-08-12

ENA last update

2018-03-08

ENA-CHECKLIST

ERC000011

External Id

SAMEA2165299

INSDC center alias

NCMLS

INSDC center name

Nijmegen Centre for Molecular Life Sciences - Radboud University

INSDC first public

2014-08-12T17:01:04Z

INSDC last update

2018-03-08T16:39:00Z

INSDC status

public

SAMPLE_ONTOLOGY_URI

http://purl.obolibrary.org/obo/CLO_0009457

Submitter Id

U-266_d01

biomaterial provider

DSMZ

biomaterial type

Cell Line

common name

human

differentiation method

Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011

differentiation stage

Plasma cell

disease

Multiple Myeloma

line

U-266

medium

RPMI 1640 + 20%FBS + 1% Glutamine

molecule

genomic DNA

sample name

U-266_d01

sex

male

Sequenced DNA Library

library_name

blueprintWP4_U266_DNaseI_60U_18M_starting cells

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

U266 cell line nuclei were isolated and briefly treated with different concentrations of DNaseI enzyme. Based on quality check 60U DNase treated sample was further processed with sucrose fractionation and purified sample was passed to the illumina library

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

43600882

Reads aligned (%)

96.0

Duplicates removed (%)

14.8

Number of peaks

57017 (qval < 1E-05)

hg19

Number of total reads

43600882

Reads aligned (%)

95.1

Duplicates removed (%)

16.8

Number of peaks

56656 (qval < 1E-05)