Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:04Z
INSDC last update
2018-03-08T16:39:00Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://purl.obolibrary.org/obo/CLO_0002767
Submitter Id
DG-75_d01
biomaterial provider
DSMZ
biomaterial type
Cell Line
common name
human
differentiation method
Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011
differentiation stage
B cell
disease
Sporadic Burkitt lymphoma
line
DG-75
medium
RPMI 1640 + 10%FBS + 1% Glutamine
molecule
genomic DNA
sample name
DG-75_d01
sex
male
Sequenced DNA Library
library_name
blueprintWP4_DG75_DNaseI_60U_18M_starting cells
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
DG75 cell line nuclei were isolated and briefly treated with different concentrations of DNaseI enzyme. Based on quality check 60U DNase treated sample was further processed with sucrose fractionation and purified sample was passed to the illumina library