Sample information curated by ChIP-Atlas

Antigen

Antigen Class
No description
Antigen
NA

Cell type

Cell type Class
Blood
Cell type
Burkitt lymphoma cells
NA
NA

Attributes by original data submitter

Sample

DISEASE_ONTOLOGY_URI
C1336077
ENA first public
2014-08-12
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2165293
INSDC center alias
NCMLS
INSDC center name
Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:04Z
INSDC last update
2018-03-08T16:39:00Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://purl.obolibrary.org/obo/CLO_0002767
Submitter Id
DG-75_c01
biomaterial provider
DSMZ
biomaterial type
Cell Line
common name
human
differentiation method
Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011
differentiation stage
B cell
disease
Sporadic Burkitt lymphoma
line
DG-75
medium
RPMI 1640 + 10%FBS + 1% Glutamine
molecule
genomic DNA
sample name
DG-75_c01
sex
male

Sequenced DNA Library

library_name
blueprint_DG75_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
NEXTflex ChIP-Seq from Bioo Scientific, 10 cycle PCR at the end and last purification is performed with magnetic beads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
58050015
Reads aligned (%)
81.7
Duplicates removed (%)
41.3
Number of peaks
1016 (qval < 1E-05)

hg19

Number of total reads
58050015
Reads aligned (%)
81.0
Duplicates removed (%)
42.8
Number of peaks
999 (qval < 1E-05)

Base call quality data from DBCLS SRA