Sample information curated by ChIP-Atlas

Antigen

Antigen Class
No description
Antigen
NA

Cell type

Cell type Class
Blood
Cell type
Multiple Myeloma
MeSH Description
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Attributes by original data submitter

Sample

DISEASE_ONTOLOGY_URI
C0026764
ENA first public
2014-08-12
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2165292
INSDC center alias
NCMLS
INSDC center name
Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:04Z
INSDC last update
2018-03-08T16:39:00Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://purl.obolibrary.org/obo/CLO_0009457
Submitter Id
U-266_c01
biomaterial provider
DSMZ
biomaterial type
Cell Line
common name
human
differentiation method
Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011
differentiation stage
Plasma cell
disease
Multiple Myeloma
line
U-266
medium
RPMI 1640 + 20%FBS + 1% Glutamine
molecule
genomic DNA
sample name
U-266_c01
sex
male

Sequenced DNA Library

library_name
blueprint_U266_H3K27ac
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
NEXTflex ChIP-Seq from Bioo Scientific, 10 cycle PCR at the end and last purification is performed with magnetic beads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
36682042
Reads aligned (%)
94.9
Duplicates removed (%)
16.4
Number of peaks
16835 (qval < 1E-05)

hg19

Number of total reads
36682042
Reads aligned (%)
94.2
Duplicates removed (%)
17.3
Number of peaks
16741 (qval < 1E-05)

Base call quality data from DBCLS SRA