Sample information curated by ChIP-Atlas

Antigen

Antigen Class
No description
Antigen
NA

Cell type

Cell type Class
Blood
Cell type
Burkitt lymphoma cells
NA
NA

Attributes by original data submitter

Sample

DISEASE_ONTOLOGY_URI
C1336077
ENA first public
2014-08-12
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2165291
INSDC center alias
NCMLS
INSDC center name
Nijmegen Centre for Molecular Life Sciences - Radboud University
INSDC first public
2014-08-12T17:01:04Z
INSDC last update
2018-03-08T16:39:00Z
INSDC status
public
SAMPLE_ONTOLOGY_URI
http://www.ebi.ac.uk/efo/EFO_0005475
Submitter Id
BL-2_c01
biomaterial provider
DSMZ
biomaterial type
Cell Line
common name
human
differentiation method
Drexler HG chapter 16 Establishment and culture of leukemia-lymphoma cell in Cancer Cell Culture Methods and Protocols 2nd Edition Springer Berlin pp 181 to 200 in 2011
differentiation stage
B cell
disease
Sporadic Burkitt lymphoma
line
BL-2
medium
RPMI 1640 + 10%FBS + 1% Glutamine
molecule
genomic DNA
sample name
BL-2_c01
sex
male

Sequenced DNA Library

library_name
blueprint_BL2_H3K4me1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
NEXTflex ChIP-Seq from Bioo Scientific, 10 cycle PCR at the end and last purification is performed with magnetic beads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
47109383
Reads aligned (%)
96.2
Duplicates removed (%)
11.2
Number of peaks
14881 (qval < 1E-05)

hg19

Number of total reads
47109383
Reads aligned (%)
95.5
Duplicates removed (%)
12.2
Number of peaks
14618 (qval < 1E-05)

Base call quality data from DBCLS SRA