ChIP-Atlas: ERX278909

Antigen

Cell type Class: Bone
Cell type: U2OS
Tissue: bone
Lineage: mesoderm
Description: osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

ENA first public: 2014-03-22
ENA last update: 2018-03-08
External Id: SAMEA2162146
INSDC center alias: 1 Universite de Toulouse ; UPS ; LBCMCP ; 118 route de Narbonne, 31062, Toulouse, France
INSDC center name: 1 Universite de Toulouse ; UPS ; LBCMCP ; 118 route de Narbonne, 31062, Toulouse, France
INSDC first public: 2014-03-22T17:00:39Z
INSDC last update: 2018-03-08T16:34:56Z
INSDC status: public
Submitter Id: E-MTAB-1241:H3K36me3 -4OHT ChIP
broker name: ArrayExpress
cell line: AsiSI-ER-U20S
common name: human
sample name: E-MTAB-1241:H3K36me3 -4OHT ChIP

library_name: H3K36me3-4OHT
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: U20S AsiSI-ER cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with antibiotics and 10% FCS (Invitrogen) at 37 C under a humidified atmosphere with 5% CO2. Cells were treated with 300 nM 4OHT for 4 hours ChIP assays were carried out according the protocol described in (Iacovoni et al, 2010). Briefly, 200 ug of chromatin was subjected to immunoprecipitation using 2 ug of a gammaH2AX antibody (Epitomics 2212-1), XRCC4 (Abcam), or Rad51 (Santa Cruz). For ChIP against PolII-S2P (Abcam) 50 ug of chromatin was used, instead of 200 ug. After the washes the beads/chromatin complexes were re-suspended in 200 uL of TE buffer (Tris 10mM pH8, EDTA 0,5mM pH8) and were directly subjected to crosslink reversal in presence of 0.5% SDS overnight at 70 C. Following crosslink reversion, all immunoprecipitated DNA and input DNA were purified by phenol/chloroform, followed by DNA precipitation. DNA amount was quantified using the Quant-iTTM kit from Invitrogen. 10ng of DNA was next used for sequencing.