Evolutionary comparison of CTCF and YY1 binding in primate lymphoblastoid cell lines
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Lung
Cell type
NCI-H1299
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Non-Small Cell
Attributes by original data submitter
Sample
ENA first public
2013-04-22
ENA last update
2018-03-08
External Id
SAMEA1877856
INSDC center alias
EFPL
INSDC center name
EFPL
INSDC first public
2013-04-22T17:03:04Z
INSDC last update
2018-03-08T16:17:58Z
INSDC status
public
Submitter Id
E-MTAB-1511:Human liver 2
broker name
ArrayExpress
common name
human
organism part
liver
sample name
E-MTAB-1511:Human liver 2
strain
HH1299
Sequenced DNA Library
library_name
Hsa-Liver-ChIP2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lymphoblast cell lines were obtained for seven primate species. All cell lines were transformed by the Epstein Barr virus except for the macaque cell lines that were transformed by a herpesvirus. Cells were grown in suspension at a confluency of 200 000-1 x106 cells/ml in RPMI1640 media supplemented with 10% Fetal Bovine Serum and 2 mM L-glutamine. C57BL/6J mice were housed in the Biological Resources Unit under UK Home Office licensing. Tissue was obtained from at least two independent males and cross-linked as described in (Schmidt, Methods,2009). Male and female human tissue samples were obtained from biopsied tissue collected at Addenbrooke's Hospital, Cambridge, and were provided by the Biobank under human tissue license 08/H0308/117. Liver tissue was also obtained from the Liver Tissue Distribution Program (NIDDK contract number N01-DK-9-2310) at the University of Pittsburgh. 1x10^8 cells were cross-linked with 1% formaldehyde as previously described (Schmidt, Methods,2009). ChIP-seq assays were performed as previously described (Schmidt, 2009). Protein-bound DNA was immunoprecipitated with antibodies against CTCF (Millipore, 07-729), or YY1 (Santa Cruz, sc-281).