Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

ENA first public

2018-04-24

ENA last update

2017-03-06

ENA-CHECKLIST

ERC000011

External Id

SAMEA103891180

INSDC center alias

Instituto Gulbenkian de Ciencia

INSDC center name

Instituto Gulbenkian de Ciencia

INSDC first public

2018-04-24T17:03:46Z

INSDC last update

2017-03-06T12:10:52Z

INSDC status

public

Submitter Id

E-MTAB-5541:Zeb1_Cb192

broker name

ArrayExpress

cell line

stem cell derived cell line

cell type

neural stem cell

common name

human

genotype

wild type genotype

growth condition

media

sample name

E-MTAB-5541:Zeb1_Cb192

Sequenced DNA Library

library_name

Zeb1_Cb192_s

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

38409384

Reads aligned (%)

98.2

Duplicates removed (%)

31.8

Number of peaks

1425 (qval < 1E-05)

hg19

Number of total reads

38409384

Reads aligned (%)

97.2

Duplicates removed (%)

33.6

Number of peaks

1321 (qval < 1E-05)